Biology, ADMET & PK

Dr. Torsten Diesinger, CSO of ACROVIS biostructures, is in charge of our Molecular Biology unit pooling comprehensive capabilities necessary for profound evaluations of ligand-target interactions. The goal is not only to review small molecules on their inhibitory potency in specifically designed subcellular assays (assay development) but also to get deep insights in their impact on the cells’ physiology. So after the synthesis of head compounds by our medicinal chemistry facility they have to pass different levels of studies in which their exact interaction modus with the potential target molecule and their influence on the affected biological system is investigated.

Subcellular Analyses by:
- Absorbance Spectrometry (UV/Vis)
- Analytical Ultracentrifugation
- Chromatography Units
- Fluorescence Polarimetry
- Fluorescence Spectroscopy
- HPLC Systems
- Isothermal Titration Calorimetry (ITC)
- Mass Spectrometry (MALDI TOF / MALDI TOF/TOF)
- Stopped Flow Unit (UV / Vis / FL)
- Surface Plasmon Resonance Unit (SPR / Biacore)
- Differential Scanning Calorimetry (DSC)
- Circular Dichroism (CD)

Cellular Analysis by:
- Electron Microscopy (SEM & TEM, CryoEM)
- Epifluorescence Microscopy
- FACS Arrays by BD Bioanalyzer
- Flow Cytometer by BD FACS Canto
- Real Time qPCR Arrays by Roche Light Cycler 480

Uniquely developed animal models express the respective target molecule in a pathophysiologically and disease specific manner. They serve as an in vivo test system able to validate the proof-of-concept with the most promising leads. So ACROVIS biostructures has the expertise to cover the complete research and development phase from target validation to specific animal experiments with the goal to find new drug candidates.